Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock

F Sadeghi; A Yadollahi; M Jafarkhani Kermani; M Eftekhari

doi:10.1016/j.jgeb.2014.12.006

Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock

J Genet Eng Biotechnol. 2015 Jun;13(1):19-23. doi: 10.1016/j.jgeb.2014.12.006. Epub 2015 Jan 31.

Authors

F Sadeghi¹, A Yadollahi², M Jafarkhani Kermani³, M Eftekhari²

Affiliations

¹ Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
² Department of Horticultural Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
³ Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, Karaj, Islamic Republic of Iran.

Abstract

The enormous demand for new rootstock genotypes in Prunus spp. makes us to use micropropagation as an unavoidable propagation method. Therefore, the study on micropropagation of a new semi-dwarf vegetative rootstock namely Tetra (Prunus empyrean 3) was carried out to develop an optimized protocol. Culture establishment using nodal segments was enhanced using WPM (woody plant medium) medium lacking growth regulators. From various shoot multiplication treatments, the highest number of shoots per explant (30.4) was found on ME (Media created specifically) medium supplemented with 0.8 mg l^-1 BAP and 0.05 mg l^-1 IBA. 100% in vitro rooting was achieved on ½ strength MS medium with 0.5 mg l^-1 IBA, 1.6 mg l^-1 thiamine and 150 mg l^-1 iron sequestrene.

Keywords: BAP, 6-benzylamino purine; DW, dry weight; FW, fresh weight; IBA, indol-3-butric acid; In vitro propagation; LS, Linsmaier and Skoog (1965); ME, media created specifically (Cos et al., 2004); MS, Murashige and Skoog (1962); Rooting; Tetra; Vegetative rootstock; WPM, woody plant medium.